Archives by Tag 'Research'

Research services

By valentova - Last updated: Pátek, Leden 28, 2011

Biomedical research services for industry, research institutes and individual research groups . Department of medical chemistry and biochemistry (DMCB) provides screening of a biologically active substance/extract/phytopreparation (on model reactions, mammalian cells and animal models) and consulting services for pharmaceutical and food industry, research institutes and individual research groups. Based on the needs of the customer, […]

Antioxidant testing

By valentova - Last updated: Pátek, Leden 28, 2011

Implication (i) Assessment of antioxidant potency Focus (i)  Scavenging of free radicals (ii) Prevention of lipid peroxidation Models (i)  Screening of potency of the test compound in vitro (ii) In vivo screening  ® demonstration of the effect on an animal model (rat, mouse) ® placebo controlled studies with human volunteers (iii) The use of oxidized LDL as bioindicators Why […]

Lipid metabolism

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i) Atherosclerosis, cardiovascular disease, injury process Focus (i) Oxidized LDL (ii) Plasma lipids and lipoproteins Models (i) Screening ®  animal model Why LDL oxidation? For several years it has been known that development of atherosclerosis is related to the level of the ”noxious” low-density lipoprotein (LDL). More recent studies have revealed that ultimate atherogenic […]

Cellular defence functions

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i) Chronic inflammations (rheumatoid arthritis, inflammatory skin diseases, psoriasis, Crohn’s disease, asthma, HIV), ischemia – reperfusion (infarction, stroke, organ transplantation) Focus (i) Production of reactive oxygen species by neutrophils and other cells of host defence Models (i) Screening  ®  demonstration of the effect in vivo (human volunteers) Why cellular defence functions? Generation of reactive […]

DNA damage

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i) Genotoxicity, carcinogenesis Focus (i) Oxidative modification of DNA Models (i) Direct (in vitro) effect  ® cell culture models  ®  animal models  ® human volunteers Why DNA damage? Significant levels of DNA damage leading to mutations and carcinogenesis are caused by endogenous oxidants. It is estimated that half of all human cancers are due […]

Effects on cytochromes P450 system

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i)   Drug-drug interactions (ii)  Carcinogenesis (iii) Cytotoxicity Focus (i)  Inhibition of specific activities of cytochromes P450 (CYP1A, 2A6, 2C9, 2D6, 2E1, 3A4) (ii) Induction/repression of CYP 1A Models (i)  CYP activities in human liver microsomes (ii) Hepatoma cell line HepG2 (alternative: primary rat hepatocytes) Why effects on CYP enzymes? Phase I biotransformation is frequently […]

Cell cytotoxicity/cytoprotectivity

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i) Biosafety (ii) In vitro toxicity Focus (i) Cytotoxicity (prooxidant activity) (ii) Cytoprotectivity (elimination of oxidative stress) Models (i) Primary cell culture (rat, pig and/or human hepatocyte; rat cardiomyocyte) (ii) Cell line (fibroblast (3T3 and Balb); keratinocyte (HaCaT); endothelial cell (HUVEC); HL-60; HepG2; HeLa) Why testing of cytotoxicity/cytoprotectivity? In vitro screening of cytotoxic properties […]

Safety evaluation on animals

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i) Biosafety (ii) In vivo toxicity (iii) Metabolization Focus (i) Safety (ii) Oxidative stress and related parameters (iii) Detection and quantification of substances and metabolites in body fluids, tissues and faeces Models (i) Experimental animals (rats, mice, pigs) Why testing safety in vivo? In vitro screening of cytotoxic properties of the test substance/extract/phytopreparation can […]

Safety evaluation on human volunteers

By valentova - Last updated: Pátek, Leden 28, 2011

Implications (i) Biosafety Focus (i) Safety (ii) Oxidative stress and related parameters (iii) Detection and quantification of substances and metabolites in body fluids Models (i) Human volunteers Why testing safety on human volunteers? Safety testing on human volunteers serves for confirmation of results obtained on experimental animals and/or cell cultures. This confirmation is necessary because […]

Grants

By valentova - Last updated: Čtvrtek, Leden 27, 2011

Grants in 2016 . The Czech Science Foundation . European Cooperation in Science and Technology . Ministry of education, youth and sports . Comprehensive sustainable systems in agriculture (Ministry of agriculture of Czech Republic) . Technology Agency of the Czech Republic . Ministry of Health of the Czech Republic